The brain is a complex functional structure which, among many things, regulates and controls both motoric and sensory functions within the body. To cope with this vast amount of processing, the brain is sourced with a complex vascular system to ensure sustained energy supply and waste management. Hemodynamics encompasses our contemporary understanding of how this system is regulated. Using the high spatiotemporal resolution of two-photon fluorescence microscopy, data from nine mice were acquired for estimating hemodynamic parameters (red blood cell velocity, diameter dilation, cell flux and cell linear density). Through a cranial window, singular capillaries were sampled over a 60 seconds window with a middle 20 seconds period of forepaw stimulation. Samplings were repeated three times with altering stimulation current, incrementing depth regions from 100μm to 400μm circumventing cortical layer IV of mice. The study yielded significant hemodynamic changes through the cortical layers. During baseline conditions, parameters displayed somewhat dependency through layers, while stimulation significant altered the baseline values for most parameters, incrementing with depth. Post-baseline conditions showed significant increased values compared to baseline predominantly in layer II-III.

DATE: Friday 19 September 2014
TIME: 10:30 - 11.30
PLACE: CFIN meeting room, 5^th floor, Aarhus University Hospital, Building 10G, Nørrebrogade 44 

ALL ARE WELCOME to attend the defense.