Lara Marziani
Biomedical sciences, University of Perugia, Italy
Sino-Danish collaboration double degree PhD program.

Title: Exploring the therapeutic potential of remote ischemic conditioning-induced miRNAs in stroke: mechanistic insight into modulating transcriptomic and functional changes in immortalized human brain microvascular endothelial cells under inflammatory conditions

Location: AUH, auditorium G206-142,
See MAP …

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Summary:

Stroke remains the second leading cause of death worldwide. Specifically, inflammation underlying stroke can aggravate secondary brain injuries and lead to functional and cognitive disabilities. Despite current therapeutic options, many patients still experience significant neurological damage, highlighting the need for new strategies that can support brain repair and functional recovery. Remote Ischemic Conditioning (RIC) is a non-invasive medical procedure, aimed at offering neuroprotection beyond the limited time window of conventional stroke treatments. The effect of RIC is thought to be mediated, at least in part, by circulating bioactive molecules including microRNAs. Emerging evidences suggest that microRNAs may help to modulate angiogenesis, de-novo formation of new blood vessels, a key process involved in post-stroke repair.

This study investigated the role of four RIC-induced microRNAs, miR-16-5p, miR-144-3p, miR-182-5p, and miR-451a, in regulating angiogenesis in immortalized human brain microvascular endothelial cells (IM-HBMECs) exposed to inflammatory conditions. Computational analysis and in vitro validation, helped to identify two angiogenesis-related genes, SLIT2 and TEK, as functional targets of miR-16-5p and miR-144-3p, respectively. Angiogenesis assays further revealed that miR-16-5p and miR-144-3p influence angiogenic behaviour in IM-HBMECs exposed to inflammatory conditions in vitro. Overall, these findings suggest that miR-16-5p and miR-144-3p may contribute to the regulation of post-stroke angiogenesis, supporting their potential relevance in stroke recovery.

After the defense CFIN is hosting a small reception just outside the auditorium.
ALL ARE WELCOME.

Assesment committee:
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• Professor Jie REN
  State Key Laboratory of RNA Innovation, Science and Engineering, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology
   
• Associate Professor Reza Khorooshi
  Neurobiology Research Unit, Department of Molecular Medicine, University of Southern Denmark
   
• Associate Professor Mai Marie Holm (chair of evaluation committee and moderator of the defence)
  Department of Biomedicine, Aarhus University

Contact:

Lara Marziani
PhD student